Comparison of homemade and imported HbsAg ELISA kits on screening blood samples.
- Author:
Fu-ping LIU
1
;
Jing-chun LIU
;
De-wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Blood Donors; China; Enzyme-Linked Immunosorbent Assay; instrumentation; methods; standards; Hepatitis B; blood; diagnosis; prevention & control; Hepatitis B Surface Antigens; blood; Humans; Mass Screening; methods; standards; Reagent Kits, Diagnostic; standards; Reference Standards; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2006;20(2):84-86
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo evaluate homemade and imported HbsAg ELISA kits on screening blood donors.
METHODSSamples for evaluation included 120 HbsAg serum plates for the golden criteria and 400 sets of serum from blood donors in Dongguan. The samples underwent blind screening with homemade and imported ELISA kits respectively.
RESULTSThe sensitivity of homemade (Xinchuang) and imported (Diasorin) HbsAg ELISA kit were 85.71% (72/84) and 100% (84/84), respectively. Their specificity was 100% (436/436) and 96.55% (421/436) respectively. The consistency of two ELISA kits was 100%.
CONCLUSIONThe imported ELISA kit had the highest sensitivity, but its specificity was not as good as that of homemade ELISA kit. The two kinds of ELISA kits had good repetition. The combination of the two reagents may ensure the safety of blood transfusion.
