Immunogenicity of a chimeric adenovirus type 5 vector with type 35 fiber containing HIV-1 gag in mice.
- Author:
Xin-lei LIU
1
;
Shuang-qing YU
;
Xia FENG
;
Xiao-li WANG
;
Hong-mei LIU
;
Xiao-mei ZHANG
;
Hong-xia LI
;
Ling ZHOU
;
Ze-lin LI
;
Yi ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: AIDS Vaccines; genetics; immunology; Adenoviridae; genetics; Animals; Enzyme-Linked Immunosorbent Assay; Female; Fluorescent Antibody Technique, Indirect; Gene Products, gag; genetics; immunology; metabolism; Genetic Vectors; genetics; HIV Antibodies; blood; HIV-1; genetics; immunology; Immunization; methods; Immunoglobulin G; blood; Mice; Mice, Inbred BALB C; Random Allocation; Recombinant Fusion Proteins; genetics; immunology; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):5-7
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the immune effect of a chimeric adenovirus type 5 vector with type 35 fiber (rAd5/F35) vaccine in BALB/c mice.
METHODSThe expression of HIV Gag protein was determined using indirect immunofluorescent staining. The rAd5/F35-mod.gag vector was injected intramuscularly to mice. The IgG antibody was detected by ELISA and CTL response was detected by intracellular cytokine stain assay.
RESULTSThe rAd5/F35-mod.gag vector could express HIV Gag protein in vitro and generate strong HIV-specific immune responses in vivo. But anti-Ad5 immunity could limit its immunogenicity in vivo.
CONCLUSIONThe rAd5/F35-mod.gag vector can elicit specific CTL response and IgG antibody in animal model. In mice with high Ad5 vector-specific immunity, Ad5/F35-mod.gag showed lower level of Gag specific CTL and antibody response than in mice without pre-existing adenovirus type 5 immunity. The results indicated that fiber exchange alone does not evade pre-existing Ad5 immunity.
