Association between human papillomavirus (HPV) type 16 infection and E6/E7 gene variant and the cervical lesions in Beijing.
- Author:
Ying-jie YANG
1
;
Jian ZHAO
;
Qin-ping LIAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; China; epidemiology; Female; Gene Frequency; Genotype; Host-Pathogen Interactions; Human papillomavirus 16; genetics; physiology; Humans; Middle Aged; Mutation; Oncogene Proteins, Viral; genetics; Papillomavirus E7 Proteins; Papillomavirus Infections; epidemiology; pathology; virology; Repressor Proteins; genetics; Severity of Illness Index; Uterine Cervical Diseases; epidemiology; pathology; virology; Uterine Cervical Neoplasms; epidemiology; pathology; virology; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):32-34
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between HPV16 infection, E6/E7 variations and the cervical lesions.
METHODSHPV subtypes were detected by using flow-through hybridization technique, E6/E7 gene was extracted from cervical lesions in 80 patients with HPV16 infection, PCR amplified, cloned into plasmid pMD18-T and sequenced.
RESULTSHPV 16 was the most common type which accounted for 33.3% (154/463), the HPV16 infection rates increased with the severity of cervical lesions (P < 0.05). Totally in 72 cases the complete E6 and E7 regions were successfully sequenced, the DNA mutation rate of E6/E7 was 88.9% (64/72). A mutation, E6-D32E (T96G) coincided with a specific type of E7 mutation, N29S (A86G). D32E/N29S mutation rate was 38.9% (28/72), the detection rate increased with the severity of cervical lesions (P < 0.05).
CONCLUSIONHPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.
