Protective effect of phloroglucinol against gamma radiation-induced oxidative stress in hair follicles.
10.14405/kjvr.2016.56.1.29
- Author:
Areum KIM
1
;
So Jin BING
;
Jinhee CHO
;
Khinm HERATH
;
You Jin JEON
;
Byung Gul LEE
;
Jae Woo PARK
;
Youngheun JEE
Author Information
1. Department of Advanced Convergence Technology & Science, Jeju National University, Jeju 64243, Korea. yhjee@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
gamma-ray;
hair follicular cells;
oxidative stress;
phloroglucinol;
radioprotection
- MeSH:
Animals;
Apoptosis;
Caspase 3;
Cell Death;
DNA Damage;
Hair Follicle*;
Hair Removal;
Hair*;
Korea;
Mice;
Oxidative Stress*;
Phaeophyta;
Phenol;
Phloroglucinol*;
Polyphenols;
Reactive Oxygen Species;
Regeneration;
Skin
- From:Korean Journal of Veterinary Research
2016;56(1):29-35
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
When exposed to gamma-rays, hair follicular cells immediately go through apoptosis, which hampers their rapid differentiation essential for the regeneration of hair. Phloroglucinol (PG) is a phenolic compound of Ecklonia cava, brown algae abundant in Jeju island, Korea. Containing plentiful polyphenols, PG is known for its instructive effects by inhibiting apoptosis, scavenging oxygen radicals, and protecting cells against oxidative stress. In this study, we demonstrate that PG rescues radiosensitive hair follicular cells from gamma radiation-induced apoptosis and DNA damage. To identify protective capacity of PG on hair follicles, we irradiated with 8.5 Gy (1.5 Gy/min) of gamma-rays to the whole body of C57BL/6 mice at day 6 after depilation with or without PG. In mice exposed to radiation, the expression of proapoptotic molecule p53 was downregulated in the skin of PG treated group. On immunohistochemical observation of the skin, PG inhibited the immunoreactivity of p53 and cleaved caspase-3. PG treatment protected hair follicular cells from cell death due to gamma-radiation. Our results suggest that PG presents radioprotective effects by inhibiting apoptosis of radiosensitive hair follicular cells and can protect hair follicular cells from gamma-ray induced damage.
