Therapeutic effect of autologous cytokine-induced killer cells on patients with liver cirrhosis caused by HBV infection.
- Author:
Hai-bin SU
1
;
Han-wei LI
;
Hong-lan ZHAO
;
Ming SHI
;
Bing ZHANG
;
Zi-rong TANG
;
Zhou-yun LEI
;
Hui-fen WANG
;
Fu-sheng WANG
Author Information
- Publication Type:Clinical Trial
- MeSH: Adoptive Transfer; adverse effects; methods; Adult; Aged; Cells, Cultured; Cytokine-Induced Killer Cells; cytology; immunology; transplantation; Fatigue; etiology; Female; Headache; etiology; Hepatitis B; complications; virology; Humans; Liver Cirrhosis; etiology; immunology; therapy; Male; Middle Aged; Transplantation, Autologous; Treatment Outcome
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):64-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the therapeutic effect of autologous cytokine-induced killer cells (CIK) on HBV DNA positive patients with liver cirrhosis.
METHODSHBV DNA positive 33 patients with cirrhosis were treated with CIK. Before and after cultured in vitro and post-treatment, CD3+, CD3+CD4+, CD3+CD8+, CD3+CD56+ cells, mDC and pDC were detected by flow cytometry. The indexes of virus and liver function were compared between pre- and post-treatment.
RESULTSCD3+, CD3+CD8+ cells and CD3+CD56+ cells were higher after cultured in vitro and after transfused back than those before culture (91.5 +/- 10.3, 74.4 +/- 9.9 vs. 67.9 +/- 12.8; 60.9 +/- 15.5, 37.3 +/- 15.1 vs. 27.9 +/- 10.9; 18.4 +/- 11.7, 14.5 +/- 7.5 vs. 10.6 +/- 7.1). The percentages of mDC and pDC also increased after-treatment vs. pre-treatment (0.54 +/- 0.18 vs. 0.70 +/- 0.29; 0.26 +/- 0.13 vs. 0.41 +/- 0.25). HBV DNA became undetectable in 12 patients and decrease exceeded 100 times in 4 patients after treatment. HBeAg became undetectable in 10 of 14 patients who were HBeAg positive pretreatment patients, among them 2 patients had HBeAb sero conversion. The liver function was improved after treatment. All patients tolerated the treatment.
CONCLUSIONCIK treatment can increase immune effector cells and has some antiviral effect and is safe.
