Subject diagnostic value of detecting a1pha-fetoprotein variants with a new microspincolumn method in hepatocellular carcinoma.
- Author:
Ai-fang XU
1
;
Miao-chan WANG
;
Dong-ming SUI
;
You-hong YUAN
;
Gang CHEN
;
Guo-qiang LOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Hepatocellular; blood; diagnosis; Diagnosis, Differential; Female; Hepatitis, Chronic; blood; diagnosis; Humans; Immunoassay; methods; Liver Cirrhosis; blood; diagnosis; Liver Neoplasms; blood; diagnosis; Luminescent Measurements; methods; Male; Middle Aged; Sensitivity and Specificity; Young Adult; alpha-Fetoproteins; analysis
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):67-69
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the usefulness of new microspincolumn method for the measurement of a1pha-fetoprotein variant AFP-L3 in differentiation of benign and malignant liver disease and the warming for liver cancer.
METHODSAFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin (LCA), AFP and AFP-L3 were determined with chemiluminescent immunoassay, the proportion of AFP-L3 levels AFP-L3(%) were calculated, and the relationship between the elevated AFP-L3(%) levels and benign and malignant liver disease was analyzed.
RESULTSThe levels of AFP-L3(%) in serum of patients with hepatocellular carcinoma was significantly higher than those in the patients with other liver diseases (P < 0.001). Taking AFP-L3(%) >or= 10% as the diagnostic criteria, the sensitivity for diagnosis of liver cancer was 90.9%.
CONCLUSIONDetection of AFP-L3 seemed to be of clinical value in diagnosis and differential diagnosis of hepatocellular carcinoma; it may be especially important for identifying patients with hepatocellular carcinoma whose a1pha-fetoprotein level is low.
