Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood.
- Author:
Yan HU
1
;
Jian-yang YANG
;
Lei ZHU
;
Jun HOU
;
Hong-hui SHEN
;
Pan-yong MAO
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; immunology; isolation & purification; Autoantibodies; immunology; isolation & purification; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay; Erythrocytes; immunology; Gene Expression; Genetic Vectors; genetics; HIV Antibodies; blood; immunology; HIV Envelope Protein gp160; genetics; immunology; metabolism; HIV Seropositivity; blood; Hemagglutination Tests; methods; Humans; Recombinant Fusion Proteins; genetics; immunology; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2007;21(1):76-78
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.
METHODSThe gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli.
RESULTSThe fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present.
CONCLUSIONThe fusion protein has the potential in rapid detection of HIV.
