Identification of differential genes in ovarian cancer using representational difference analysis of cDNA.
- Author:
Hong CHEN
1
;
Min WANG
;
Xin-Yan WANG
;
Shan GAO
;
Jun WANG
;
Xiao-Ming GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; analysis; genetics; Base Sequence; Cystadenocarcinoma, Serous; genetics; DNA, Complementary; genetics; DNA, Neoplasm; genetics; Female; Gene Expression Profiling; Genes, Tumor Suppressor; Humans; Microfilament Proteins; analysis; genetics; Molecular Sequence Data; Muscle Proteins; analysis; genetics; Ovarian Neoplasms; genetics; Ovary; chemistry; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid
- From: Chinese Medical Sciences Journal 2005;20(3):185-189
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify differential genes between normal ovarian epithelium tissue and ovarian epithelial cancer using representational difference analysis of cDNA (cDNA-RDA).
METHODScDNA-RDA was performed to identify the differentially expressed sequences between cDNAs from cancer tissue and cDNAs from normal ovarian tissue in the same patient who was in the early stage of ovarian serous cystadenocarcinoma. These differentially expressed fragments were cloned and analyzed, then sequenced and compared with known genes.
RESULTSThree differentially expressed cDNA fragments were isolated using cDNA from normal ovarian tissue as tester and cDNA from cancer tissue as driver amplicon by cDNA-RDA. DP III-1 and DP III-2 cDNA clone showed significant homology to the cDNA of alpha actin gene; DP III-3 cDNA clone showed significant homology to the cDNA of transgelin gene.
CONCLUSIONcDNA-RDA can be used to sensitively identify the differentially expressed genes in ovarian serous cystadenocarcinoma. Ovarian serous cystadenocarcinoma involves alteration of multiple genes.
