Expression and significance of Survivin mRNA in lung cancer tissue microarray detected by FISH.
- Author:
Xin-Yun WANG
1
;
Zhi YAO
;
Yan LI
;
Ting LIU
;
Hai-Yan ZHENG
;
Cong-Zhong ZHU
;
Cui-Yun SUN
;
Ai-Xiang WANG
;
Min ZHAO
;
Xing-Ye WU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; secondary; Adult; Aged; Carcinoma, Squamous Cell; metabolism; secondary; Female; Gene Expression Regulation, Neoplastic; Humans; In Situ Hybridization, Fluorescence; Inhibitor of Apoptosis Proteins; Lung Neoplasms; metabolism; pathology; Lymph Nodes; pathology; Lymphatic Metastasis; Male; Microtubule-Associated Proteins; biosynthesis; genetics; Middle Aged; Neoplasm Proteins; biosynthesis; genetics; Neoplasm Staging; RNA, Messenger; biosynthesis; genetics; Tissue Array Analysis
- From: Chinese Medical Sciences Journal 2005;20(3):214-216
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the expression of Survivin mRNA in lung cancer tissue microarray (TMA) by fluorescence in situ hybridization (FISH) method, and determine the role and significance of it in lung cancer genesis and progress.
METHODSThe expression of Survivin mRNA was detected by FISH method and TMA technology. Fifty-four cases of lung cancer and 10 cases of normal lung tissue were examined.
RESULTSSurvivin mRNA was expressed in 66.7% (36/54) of lung cancer; the positive ratio of lung cancer was significantly higher than that of normal lung tissue (0/10; chi2 = 15.238, P < 0.05). The positive ratio of Survivin mRNA was significantly higher in poor differentiated cancer (20/24, 83.3%) than moderate and well differentiated cancer (16/30, 53.3%; chi2 = 5.40, P < 0.05). The positive ratio of Survivin mRNA was significantly higher in group with lymph node metastasis (27/32, 84.4%) than without lymph node metastasis (9/22, 40.9%; chi2 = 11.084, P < 0.05). The positive ratio of Survivin mRNA was significantly higher in stage III-IV(12/13, 92.3%) than stage I - II (24/41, 58.5%; chi2 = 5.066, P < 0.05).
CONCLUSIONSurvivin mRNA highly expresses in lung cancer, which is related to the progress and malignant behavior. Survivin may play a promoting role in lung cancer genesis and progress and provide a basis for estimating prognosis and treatment.
