Allele distribution of five X-chromosome short tandem repeat loci in Ewenke population of north China.
- Author:
Shan-zhi GU
1
;
Teng CHEN
;
Qing-bo LIU
;
Bing YU
;
Sheng-bin LI
Author Information
- Publication Type:Journal Article
- MeSH: Asian Continental Ancestry Group; China; ethnology; Chromosomes, Human, X; genetics; DNA; genetics; Female; Forensic Medicine; Gene Frequency; Genetic Markers; Humans; Male; Microsatellite Repeats; Polymerase Chain Reaction; Polymorphism, Genetic
- From: Chinese Medical Sciences Journal 2005;20(4):237-241
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the allele genetic polymorphism of five short tandem repeat (STR) loci on X-chromosome in Ewenke population of north China and to provide basic data for forensic identification.
METHODSGenomic DNA was extracted from EDTA-whole blood of Ewenke population by Chelex-100. The DNA samples were amplified by PCR and were analyzed by polyacrylamide gel electrophoresis and silver staining. The sequence length variations of DXS6799, DXS8378, DXS101, HPRTB, and DXS6789 loci on X-chromosome in 98 unrelated Ewenke individuals were investigated.
RESULTSAll five loci analyzed showed high polymorphism and genetic stability. The data of the five X-chromosome STR loci in Ewenke ethnic group of China was in accordance with Hardy-Weinberg equilibrium by Chi-square test.
CONCLUSIONAllele polymorphism of five X-chromosome STR loci can be used as a genetic marker for forensic identification and population genetic research.
