Expression and switching of TH1/TH2 type cytokines gene in human gliomas.
- Author:
Gang LI
1
;
Yong-sheng HU
;
Xin-gang LI
;
Qing-lin ZHANG
;
Dong-hai WANG
;
Song-feng GONG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Astrocytoma; metabolism; pathology; Brain Neoplasms; metabolism; pathology; Cell Line, Tumor; Child; Child, Preschool; Cytokines; metabolism; Female; Gene Expression Regulation, Neoplastic; Glioblastoma; metabolism; pathology; Humans; Interferon-gamma; metabolism; Interleukin-10; metabolism; Interleukin-2; metabolism; Interleukin-4; metabolism; Interleukin-6; metabolism; Male; Middle Aged; Th1 Cells; metabolism; Th2 Cells; metabolism
- From: Chinese Medical Sciences Journal 2005;20(4):268-272
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas.
METHODSInterleukin (IL)-2 and interferon-gamma represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Thl/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA).
RESULTSThe total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues.
CONCLUSIONThere is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Thl/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.
