Detection of pathogens causing genital ulcer disease by multiplex polymerase chain reaction.
- Author:
Ai-ying LIU
1
;
Ming-jun JIANG
;
Yue-ping YIN
;
Jiang-fang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Chlamydia trachomatis; genetics; isolation & purification; DNA Primers; Haemophilus ducreyi; genetics; isolation & purification; Herpesvirus 1, Human; genetics; isolation & purification; Herpesvirus 2, Human; genetics; isolation & purification; Humans; Polymerase Chain Reaction; methods; Sexually Transmitted Diseases; complications; microbiology; virology; Treponema pallidum; genetics; isolation & purification; Ulcer; complications; microbiology; virology
- From: Chinese Medical Sciences Journal 2005;20(4):273-275
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo establish a multiplex polymerase chain reaction (M-PCR) assay for simultaneous detection of pathogens causing genital ulcer disease (GUD).
METHODSBased on the gene-specific region of the following pathogens: Chlamydia trachomatis omp1/ompb, herpes simplex virus (HSV) DNA polymerase, Treponema pallidum tpp47, Haemophilus ducreyi 16s rRNA, four sets of primers were designed and an M-PCR assay was developed to detect four pathogens in one test. The assay was evaluated with diagnostic result of golden standard for each pathogen.
RESULTSOf the 51 clinical samples, M-PCR showed slightly higher positive rate (47.1%) of HSV than cell culture (23.6%). Meanwhile, the positive rate of T. pallidum detected by M-PCR and dark-field microscopy was 19.6% (10/51) and 15.7% (8/51), respectively. Only one sample was positive for H. ducreyi and no sample was positive for C. trachomatis detected by both M-PCR assay and culture.
CONCLUSIONThis primary study indicated that M-PCR assay can simultaneously and rapidly detect the four etiologic pathogens causing GUD.
