Islet formation and regeneration.
- Author:
Kai-ming YANG
1
;
Ai-dong LI
;
Yan MEI
;
Hong-ying ZHOU
;
Hua LI
;
Hui-jun YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Diabetes Mellitus, Experimental; chemically induced; metabolism; pathology; Embryonic Development; physiology; Epithelial Cells; cytology; physiology; Humans; Insulin-Secreting Cells; cytology; physiology; Islets of Langerhans; cytology; physiology; Male; Pancreas; cytology; embryology; physiology; Pancreatic Ducts; cytology; embryology; physiology; Rats; Rats, Sprague-Dawley; Regeneration; physiology; Stem Cells; cytology; metabolism; physiology
- From: Chinese Medical Sciences Journal 2006;21(1):27-32
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the mechanisms of differentiation and development of pancreatic endocrine cells as well as pancreatic regeneration.
METHODSHuman embryonic pancreatic tissue at 7-14 weeks of gestation was collected. Diabetes mellitus rat model was induced with 65 mg/kg of streptozotocin. Insulin, glucagon, somatostatin, nestin, and cytokeratin 19 (CK19) of pancreatic tissues were observed by immunohistochemistry.
RESULTSAt 9 weeks of gestation, pancreatic epithelial cells began to co-express insulin, glucagon, somatostatin, and CK19 before migration. Islet cells gradually congregated along with the increase of aging, and at 14 weeks of gestation histological examination showed islet formation. At 12 weeks of gestation, nestin-positive cells could be seen in the pancreatic mesenchyme. During early embryogenesis, islet cells of pancreatic ducts co-expressed insulin, glucagon, and somatostatin. During pancreatic regeneration after damage, nestin expression of islet cells increased.
CONCLUSIONIn the early stage of embryogenesis, islet cells of primary pancreatic ducts can be differentiated to multipotential endocrine cells before migration. During tissue regeneration, pancreatic stem cells may differentiate and proliferate to form pancreatic islet.
