In vitro and in vivo study of fluorescent probe PLGA particles prepared by premix membrane emulsification method.
- Author:
Tao HU
;
Fei-Yan SHI
;
Lin-Mei PAN
;
Hua-Xu ZHU
;
Li-Wei GUO
- Publication Type:Journal Article
- MeSH:
Animals;
Drug Carriers;
chemistry;
Drug Delivery Systems;
instrumentation;
Emulsions;
chemistry;
Fluorescent Dyes;
chemistry;
Lactic Acid;
chemistry;
Mice;
Mice, Nude;
Nanoparticles;
chemistry;
Particle Size;
Polyglycolic Acid;
chemistry
- From:
China Journal of Chinese Materia Medica
2014;39(23):4583-4589
- CountryChina
- Language:Chinese
-
Abstract:
Relatively uniform-sized nanoparticles made of poly (lactic-co-glycolic acid) (PLGA) were prepared by premix membrane emulsification method. After the drug loading property was completed, the dynamic tissue distribution of nanoparticles was recorded. With the average particle size and span as indexes, membrane pore size, number of passing membrane times, membrane pressure, volume ratio of oil-water phase and the concentration of poly(vinyl alcohol) (PVA) in external water phase were investigated by single factor test, the optimum preparation technology of blank PLGA nanlparticles was as following: pore size of SPG membrane was 1 μm, membrane pressure was 1. 15 MPa, the number of passing membrane time was 3, the mass fraction of PVA of 2%, volume ratio of oil-water phase of 1 : 5. Prepared nanoparticles were round with smooth surface, the mean diameter was 332.6 nm, span was 0.010, the confocal laser scanning microscope (CLSM) concluded that fluorescent substance is uniform composizion in PLGA nanoparticle, and the in vivo imaging technology in mice include that the nanoparticles show good liver and spleen targeting property.
