Expression profiles analysis of two member of squaleneepoxidase gene family from Eleutherococcus senticosus.
- Author:
Yue-Hong LONG
;
Fei-Fei LI
;
Guo YANG
;
Zhao-Bin XING
- Publication Type:Journal Article
- MeSH:
Eleutherococcus;
chemistry;
enzymology;
genetics;
growth & development;
Gene Expression Regulation, Plant;
Peroxidase;
genetics;
metabolism;
Plant Proteins;
genetics;
metabolism;
Saponins;
analysis;
metabolism;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2015;40(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
In order to find the characteristics of two members of gene family of squaleneexpoxidase (SE) , a quantitative real time PCR method was developed to analyze the expression of Eleutherococcus senticosus SE1 and SE2 gene from different growth periods and in different organs. The result indicated that all the expression of SE2 more than SE1 in the whole growth period and organs of E. senticosus. And in the whole growth period, expression of SE1 showed a low-high-low characteristic. Both expression of SE2 and growth period showed the same trend. The lowest content of the expression was in the roots. SE1 expression have been improved more than SE2 when treated with MeJA. The expression of E. senticosus SE1 and saponins content had significantly positive correlation (P < 0.05) and the correlation coefficients was 0. 858, while the correlation was not significant for SE2. That indicated that SE1 played a key enzyme gene in the biosynthesis of triterpenoidsaponins
