Development of Tripterygium glycosides nano-carries based on "nanoemulsion-gels" and its pharmacodynamics.
- Author:
Qing GU
;
Ben-Ming YOU
;
Di-Shun YANG
;
Yong-Hua PAN
;
Wei SHU
;
Ji-Yong LIU
- Publication Type:Journal Article
- MeSH:
Animals;
Chemistry, Pharmaceutical;
instrumentation;
methods;
Dermatitis;
drug therapy;
immunology;
Drug Carriers;
chemistry;
Drugs, Chinese Herbal;
chemistry;
pharmacokinetics;
Emulsions;
chemistry;
Female;
Glycosides;
chemistry;
pharmacokinetics;
Humans;
Interleukin-4;
immunology;
Interleukin-8;
immunology;
Mice;
Mice, Inbred ICR;
Nanoparticles;
chemistry;
Tripterygium;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(1):73-78
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to develop the Tripterygium glycosides nanoemulsion gels and investigate its pharmacodynamics. Oleic acid was used as oil phase, polyoxyethylene castor oil as surfaetant, and 1,2-propanediol as cosurfactant to screen the formula of Tripterygium glycoside nanoemulsion using the pseudo-temary phase diagrams. Then the nanoemulsion gels was prepared. The ICR mouse ears were sensitazated by 7% DNCB, and then were excited by 0.3% DNCB to stimulate the model of mouse chronic dermatitis and eczema. The concentrations of IFN-γ, IL-4 and IL-8 in mouse blood were determined by ELISA. The results showed that Tripterygium glycosides nanoemulsion gels could significantly inhibit the swelling of mouse ears(P < 0.01) and ameliorate the edama and erythema of model mouse ears skin. Also it could significantly decrease the expression of IFN-γ and IL-4 in model mouse blood. Tripterygium glycosides nanoemulsion gels had a good therapeutic effect on mouse model of dermatitis and eczema. It was expected to provide a new and long-acting exterernal preparation for the treatment of dermatitis and eczema.
