Simultaneous determination of flavones and saponins of Rhizoma Anemarrhenae by HPLC-DAD-ELSD.
- Author:
Xiao-Nan SU
;
De JI
;
Ya-Ping ZHOU
;
Li-Jun WANG
;
Wen-Yi ZANG
;
Chun-Qin MAO
;
Tu-Lin LU
- Publication Type:Journal Article
- MeSH:
Anemarrhena;
chemistry;
Chromatography, High Pressure Liquid;
instrumentation;
methods;
Drugs, Chinese Herbal;
analysis;
Flavones;
analysis;
Rhizome;
chemistry;
Saponins;
analysis
- From:
China Journal of Chinese Materia Medica
2015;40(1):108-111
- CountryChina
- Language:Chinese
-
Abstract:
This study is to establish an HPLC-DAD-ELSD method for simultaneous determination of 5 flavones and saponins in Rhizoma Anemarrhenae including neo-mangiferin, mangiferin, timosaponin B II, timosaponin B III and timosaponin A III. Samples were analyzed on a Merck Purospher STAR column(4.6 mm x 250 mm, 5 μm). The mobile phase consisted of acetonitrile( A) and 0. 1% formic acid (B) with gradient elution at a flow rate of 1.0 mL · min(-1). The column temperature was set at 40 °C. The DAD detector wavelength was set at 254 nm. The ELSD conditions were as follows: the nebulizing gas flow rate was 2.0 L · min(-1) and temperature of drift tube was 105 °C. The volume was 10 μL. The five compounds were well separated with good linear correlations. The mean recoveries were between 102.0%-104.0%. This method was quick and reliable which provides a foundation for quality control of R. Anemarrhenae.
