Flavonoids of Echinps latifolius suppress Wnt signaling in adjuvant arthritis rats.
- Author:
Cheng-Gui MIAO
;
Jian XU
;
Hu GAO
;
Liang-Liang LIU
;
Guo-Liang ZHOU
;
Mei-Song QIN
;
Jian-Zhong CHEN
;
Cheng-Feng LI
- Publication Type:Journal Article
- MeSH:
Animals;
Arthritis, Experimental;
drug therapy;
genetics;
metabolism;
Asteraceae;
chemistry;
Disease Models, Animal;
Down-Regulation;
drug effects;
Drugs, Chinese Herbal;
administration & dosage;
Flavonoids;
administration & dosage;
Humans;
Intercellular Signaling Peptides and Proteins;
genetics;
metabolism;
Male;
Membrane Proteins;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
drug effects;
Synovial Membrane;
drug effects;
metabolism;
Wnt Signaling Pathway;
drug effects;
beta Catenin;
metabolism
- From:
China Journal of Chinese Materia Medica
2015;40(1):129-133
- CountryChina
- Language:Chinese
-
Abstract:
The role of flavonoids of Echinps latifolius (FELT) in Wnt signaling was investigated in adjuvant arthritis (AA) rats. The therapeutic effects of FELT on AA rats were detected by rat arthritis score and MTT. The effect of FELT gavage treatment on the Wnt signaling key gene β-catenin, C-myc and cyclin D1 in synovium from AA rats was detected by Real-time qPCR, and the effects of FELT gavage treatment on the upstream negative regulation gene SFRP 1,2,4,5 in synovium from AA rats were detected by Real-time qPCR. The results showed that FELT gavage treatment significantly inhibited arthritis score and MTT values in AA rats, significantly inhibited the expression of the Wnt signaling gene β-catenin, C-myc and cyclin D1, significantly up-regulated the expression of the up- stream negative regulation gene SFRP 1,2,4. FELT has a better therapeutic effect for AA rats.
