Selection of reference genes of Siraitia grosvenorii by real-time PCR.
- Author:
Dong-ping TU
;
Chang-ming MO
;
Xiao-jun MA
;
Huan ZHAO
;
Qi TANG
;
Jie HUANG
;
Li-mei PAN
;
Rong-chang WEI
- Publication Type:Journal Article
- MeSH:
Cucurbitaceae;
genetics;
RNA, Ribosomal, 18S;
genetics;
Real-Time Polymerase Chain Reaction;
methods;
Reference Standards
- From:
China Journal of Chinese Materia Medica
2015;40(2):204-209
- CountryChina
- Language:Chinese
-
Abstract:
Siraitia grosvenorii is a traditional Chinese medicine also as edible food. This study selected six candidate reference genes by real-time quantitative PCR, the expression stability of the candidate reference genes in the different samples was analyzed by using the software and methods of geNorm, NormFinder, BestKeeper, Delta CT method and RefFinder, reference genes for S. grosvenorii were selected for the first time. The results showed that 18SrRNA expressed most stable in all samples, was the best reference gene in the genetic analysis. The study has a guiding role for the analysis of gene expression using qRT-PCR methods, providing a suitable reference genes to ensure the results in the study on differential expressed gene in synthesis and biological pathways, also other genes of S. grosvenorii.
