Anti-complementary phenolic acids from Lonicera japonica.
- Author:
Fu-yong NI
;
Lu LIU
;
Ya-ling SONG
;
Xue-jing WANG
;
Yi-wu ZHAO
;
Wen-zhe HUANG
;
Zhen-zhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- MeSH: Complement Inactivating Agents; chemistry; isolation & purification; pharmacology; Hydroxybenzoates; chemistry; isolation & purification; pharmacology; Lonicera; chemistry
- From: China Journal of Chinese Materia Medica 2015;40(2):269-274
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-complementary phenolic acids from Lonicera japonica.
METHODThe anti-complementary activity-directed isolation was carried out with the hemolysis test as guide. All isolation was evaluated for their in vitro anti-complementary activities. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR, 13C-NMR data.
RESULTFourteen compounds were isolated from the EtOAc fraction of L. japonica extracts, including 8 phenolic acids: 5-O-caffeoylquinic acid (1), chlorogenic (2), 4-O-caffeoylquinic acid (3), 3,5-di-O-caffeoylquinic acid (4), 4,5-di-O-caffeoylquinic acid (5), 3,4-di-O-caffeoylquinic acid (6), caffeic acid (7) and methyl caffeate acid (8); 3 iridoids: secologanoside (9), sweroside (10) and secoxyloganin (11); and 3 flavonoids: luteolin (12), quercetin (13) and kaempferol (14). Compounds 1-9 and 11-14 showed anti-complementary activity in different extents and 3,5-di-O-caffeoylquinic acid (4) exhibited the most significant activity against the classical pathway.
CONCLUSIONCompound 14 is obtained from this plant for the first time, phenolic acids are the main anti-complementary constituents of L. japonica and 3,5-di-O-caffeoylquinic acid(4) is a potential complement inhibitor with strong activity, which worthy to be studied further in the future.