Co-expression of gpd1 and hor2 from Saccharomyces cerevisiae in Escherichia coli.
- Author:
Li-Qin DU
1
;
Yu-Tuo WEI
;
Fa-Zhong CHEN
;
Zhao-Fei LUO
;
Ri-Bo HUANG
Author Information
1. Institute of Fermentation and Enzyme engineering, GuangXi University, Nanning 530005, China.
- Publication Type:Journal Article
- MeSH:
Cloning, Molecular;
Escherichia coli;
genetics;
metabolism;
Fermentation;
Fungal Proteins;
biosynthesis;
genetics;
Genetic Engineering;
Glycerol;
metabolism;
Glycerolphosphate Dehydrogenase;
biosynthesis;
genetics;
Phosphoric Monoester Hydrolases;
biosynthesis;
genetics;
Saccharomyces cerevisiae;
enzymology;
genetics
- From:
Chinese Journal of Biotechnology
2005;21(3):385-389
- CountryChina
- Language:Chinese
-
Abstract:
Based on the principle of the pathway engineering, a novel pathway of producing glycerol was built in E. coli. The gpd1 gene encoding glycerol 3-phosphate dehydrogenase and the hor2 gene encoding glycerol 3-phosphatase were cloned from Saccharomyces cerevisiae, respectively. The two genes were inserted into expression vector pSE380 together. A recombinant plasmid pSE-gpd1-hor2 containing polycistron was constructed under the control of the strong trc promoter. Then it was transformed into E. coli BL21. The result showed the recombinant microorganism GxB-gh could convert glucose to glycerol directly. And the recombinant microorganism GxB-gh was incubated to produce glycerol from D-glucose in the fermentor. The maximal concentration of glycerol was 46.67g/L at 26h. Conversion rate of glucose was 42.87%. The study is about "green" producing glycerol by recombinant microorganism and is also useful for further working in recombining microorganism of producing 1,3-propanediol.