Red/ET recombination and its biomedical applications.
- Author:
Jun-Ping WANG
1
;
You-Ming ZHANG
Author Information
1. State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, The Third Military Medical University, Chongqing 400038, China. wangjunp@yahoo.com
- Publication Type:Journal Article
- MeSH:
Bacteriophage lambda;
genetics;
DNA, Recombinant;
genetics;
DNA-Binding Proteins;
genetics;
Escherichia coli;
genetics;
metabolism;
Escherichia coli Proteins;
genetics;
Exodeoxyribonucleases;
genetics;
Genetic Engineering;
methods;
Rec A Recombinases;
genetics;
Recombination, Genetic;
genetics
- From:Chinese Journal of Biotechnology
2005;21(3):502-506
- CountryChina
- Language:Chinese
-
Abstract:
Red/ET recombination, a powerful homologous recombination system based on the Red operon of lambda phage or RecE/ RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E. coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
