Oriented immobilization of human IgG by protein A on imaging ellipsometry biosensor.
- Author:
Yan-Li MENG
1
;
Zhan-Hui WANG
;
Gang JIN
Author Information
1. National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China.
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
methods;
Humans;
Immunoassay;
methods;
Immunoglobulin G;
chemistry;
Staphylococcal Protein A;
chemistry
- From:
Chinese Journal of Biotechnology
2004;20(1):111-114
- CountryChina
- Language:Chinese
-
Abstract:
The biosensor based on optical imaging ellipsometry, can be used to detect directly, without labeling, the surface concentration of biomolecules on solid surface. The feasibility of using protein A to immobilize antibody on the silicon surface of the imaging ellipsometry biosensor was investigated in this study. The results showed that the anti-IgG immobilized by the protein A on silicon surface could bind effectively human IgG, and the human IgG immobilized on silicon surface by protein A bound more polyclonal antibody molecules than that immobilized on silicon surface directly, suggesting that protein A might block the surface to prevent the absorption of human IgG on surface directly, which might compromise its native configuration. The silicon surface modified with protein A is expected to be used to immobilize a variety of antibodies, as protein A can bind selectively the Fc regions of many mammalian IgG. The combination of imaging ellipsometry and the protein A surface modification has the potential to be developed into immunoassays of high sensitivity.
