Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes.
- Author:
Chun-Mei ZHANG
1
;
Jie QIU
;
Xiao-Hui CHEN
;
Bin WANG
;
Min ZHANG
;
Xi-Rong GUO
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; Adipocytes; drug effects; secretion; Adiponectin; secretion; Animals; Carrier Proteins; genetics; physiology; Glucose; metabolism; Glucose Transporter Type 4; analysis; metabolism; Insulin; pharmacology; Interleukin-6; secretion; Mice; Resistin; analysis; Transfection; Tumor Necrosis Factor-alpha; secretion
- From: Chinese Journal of Contemporary Pediatrics 2009;11(10):846-849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes.
METHODS3T3-L1 preadipocytes transfected with either an empty expression vector (pcDNA3.1; control group) or an NYGGF4 expression vector (NYGGF4-pcDNA3.1) were cultured in vitro and differentiated into the matured adipocytes with the standard insulin plus dexamethasone plus 3-isobutyl-methylxanthine (MDI) induction cocktail. 2-deoxy-D-[3H] glucose uptake was determined by liquid scintillation counting. Western blot was performed to detect the protein content and translocation of glucose transporter 4 (GLUT4). The supernatant concentrations of TNF-alpha, IL-6, adiponectin and resistin were measured using ELISA.
RESULTSNYGGF4 over-expression in 3T3-L1 adipocytes reduced insulin-stimulated glucose uptake. NYGGF4 over-expression impaired insulin-stimulated GLUT4 translocation without affecting the total protein content of GLUT4. The concentrations of TNF-alpha, IL-6, adiponectin and resistin in the culture medium of 3T3-L1 transfected with NYGGF4 were not significantly different from those in the control group.
CONCLUSIONSNYGGF4 over-expression impairs the insulin sensitivity of 3T3-L1 adipocytes through decreasing GLUT4 translocation and had no effects on the secretory function of adipocytes.
