Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura.
- Author:
Shao-Min REN
1
;
Chun-Xia ZHANG
;
Wei-Cai LI
Author Information
- Publication Type:Journal Article
- MeSH: Child; Child, Preschool; Female; GATA3 Transcription Factor; genetics; Humans; Interferon-gamma; blood; Interleukin-4; blood; Male; Purpura, Thrombocytopenic, Idiopathic; immunology; RNA, Messenger; blood; T-Box Domain Proteins; genetics; Th1 Cells; immunology
- From: Chinese Journal of Contemporary Pediatrics 2010;12(1):29-31
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-gamma and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP.
METHODSBlood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-gamma and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls.
RESULTSBlood T-bet mRNA expression and IFN-gamma levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (p<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (p<0.01).
CONCLUSIONSThe high expression of T-bet and IFN-gamma and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.
