Resistin inhibits rat insulinoma cell RINm5F proliferation.
- Author:
Feng LIU
1
;
Jie QIU
;
Chun-Mei ZHANG
;
Chen-Bo JI
;
Xi-Rong GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Cell Proliferation; Insulinoma; pathology; Pancreatic Neoplasms; pathology; Phosphorylation; Proto-Oncogene Proteins c-akt; metabolism; Rats; Resistin; genetics; physiology; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins; genetics; Transfection
- From: Chinese Journal of Contemporary Pediatrics 2010;12(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEResistin was thought to link the obesity to type 2 diabetes. This study aimed to investigate the effect of resistin on insulinoma cell proliferation.
METHODSpcDNA3.1-resistin was transfected into rat insulinoma cells RINm5F. Cell proliferation was assessed by the MTT assay. The resistin and SOCS3 mRNA levels were assessed by RT-PCR. The total Akt level and the phosphorylation status were assessed by Western blot.
RESULTSThe over-expressed resistin inhibited the RINm5F cell proliferation (p<0.05). SOCS-3 expression was up-regulated by resistin over-expression (3.2 folds over the control; p<0.05). Akt phosphorylation was down-regulated by resistin over-expression (0.6 fold over the control; p<0.05).
CONCLUSIONSResistin impairs the rat insulinoma cell RINm5F proliferation. This might be attributed to a down-regulation of Akt level caused by increased SOCS-3 expression.
