The antiviral role of Toll-like receptor 3 in human lung epithelial cells infected with respiratory syncytial virus
10.3760/cma.j.issn.1003-9279.2012.02.018
- VernacularTitle:呼吸道合胞病毒感染人肺上皮细胞活化Toll样受体3介导的抗病毒作用研究
- Author:
Bao-Shun YANG
1
;
Li-Jing WANG
Author Information
1. 浙江温岭市第一人民医院
- Keywords:
Respiratory syncytial virus,human;
Receptors,virus;
Interferon type Ⅰ
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(2):130-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to understand the production mechanism of interferon and provide a scientific basis for preventionand clinical therapy,the expression changes of Toll-like receptor (TLR3) mRNA and the role of TLR3 in human lung epithelial cells ( A549 cells) infected with respiratory syncytial virus (RSV) were investigated in this study.Methods RSV infected A549 cells were treated with or without specific antibodies of TLR3 and collected at the selected timepoints after RSV infection (4,8,12,16 and 24h).The expressions of TLR3,IFN-α,IFN-β and RSV F mRNA were evaluated by RT-PCR.Result It was found that RSV infection could markedly up-regulate the mRNA expression of TLR3,IFN-α,IFN-β and RSV F protein in a time-dependent manner as the 24h mRNA expressions of them were 4 times,3 times,3 times and 0.7 times mor than the basic expression,respectively.Treatment of TLR3 specific antibodies,whereas,significantly down-regulated the activation of TLR3.The mRNA expression of IFN-α and IFN-β also decreased accordingly and that of IFN-β reduced more obviously than IFN-α,but that of RSV F protein rose significantly.Conclusion Above data indicate that RSV infection could induce an apparent increase of antiviral genes of IFN-α and IFN-β by activating TLR3 in human lung epithelial cells and the activated cells mediated Type Ⅰ interferon is antiviral,which suggesting that TLR3 might play an important role in antiviral activity of RSV-infected human lung epithelial cells.
