Primary establishment of an alphaLISA assay for detection of HAV IgM.
- Author:
Xing GAN
1
;
Ying WANG
;
Juan SONG
;
Yu CUI
;
Peng SUN
;
Chen GAO
;
Chao-Ping LI
;
Jun HAN
Author Information
- Publication Type:Journal Article
- MeSH: Biotinylation; Hepatitis A; diagnosis; Hepatitis A Antibodies; blood; Humans; Immunoglobulin M; blood; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(2):139-141
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an AlphaLISA method for detection of antibody of Hepatitis A virus.
METHODSAfter hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.
RESULTSThe sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%.
CONCLUSIONA homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.
