Primary establishment of an alphaLISA assay for detection of HAV IgM
10.3760/cma.j.issn.1003-9279.2012.02.021
- VernacularTitle:甲型肝炎病毒IgM AlphaLISA检测方法的初步建立
- Author:
Xing GAN
1
;
Ying WANG
;
Juan SONG
;
Yu CUI
;
Pang SUN
;
Chen GAO
;
Chao-Ping LI
;
Jun HAN
Author Information
1. 皖南医学院
- Keywords:
Hepatitis A,virus;
Immunogloblin M;
Monitoring,immunologic
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(2):139-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an AlphaLISA method for detection of antibody of Hepatitis A virus.Methods After hepatitis A virus antigen was concentrated and biotinylated,optimal biotinylated HAV antigen,donor bead,acceptor bead concentration have been explored and determined by both dotblotting and AlphaLISA methods.97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.Results The sensitivity and specificity for anti-HAV IgM were 78％ and 98.5％,the positive and negative predictive value were 95％ and 93％.Conclusion A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.
