Study on hepatitis C virus genotypes in Yantai district, Shandong province
10.3760/cma.j.issn.1003-9279.2012.04.006
- VernacularTitle:山东烟台地区HCV感染的基因分型研究
- Author:
Guo-Ying ZHANG
1
;
Sheng-Bo PU
;
Xue-Ying YU
;
Jian YANG
;
Jian-Feng SHI
;
Ai-Ling XU
;
Zhen-Hua GUO
;
Ming-Gang QIAO
;
Jie ZHANG
;
Yong-Zheng JING
Author Information
1. 青岛大学医学院
- Keywords:
Hepatitis viruses;
Genotype;
Sequence analysis;
Phylogeny
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(4):259-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the hepatitis C virus (HCV) genotype distribution in Yantai district of Shandong province,and to explore whether the HCV genotypes was relevant to the injure of liver through the index of liver function.Methods Using specific PCR primers to amplify the HCV RNA 5' UTR/NS5B,then PCR products were sequenced by genetic analyzer.The genotypes were identified by alignment to the GenBank reference sequences and construction the phylogenetic tree of 5 ' UTR.Results Among 9 unpaid blood donors we detected two kinds of genotypes of 1b and 3a,respectively,8 cases (88.9％) and 1 case (11.1％).Among 33 cases of hepatitis C patients we detected the lb,2a and 6a the three kinds of genotypes,respectively,22 (66.7％),10 (30.3％ ) and 1 (3.03％) cases.Subtype 1b is the advantage of popular genotype in HCV carriers from Yantai district,and the distribution of 1b was no significant difference in the different population (x2 =0.796,P =0.373 ) ; The difference of indicator of liver damage in the different genotypes of subjects were significant ( P ＜ 0.05 ),the mean of ALT,AST of 2asubtype carriers was significantly higher than the 1 b-subtype population.Conclusions The genetic diversity of HCV in Shandong Yantai district was presented.The main genotypes were 1b-subtype,and 3a and 6asubtype was detected firstly.The genotype of HCV were relevant to the liver damage indicators,2a-subtype hepatitis C virus infection in the liver cells may play an important role in the disease process.
