Genetic characterization of haemagglutinin of novel H1N1 influenza viruses in Suzhou City.
- Author:
Wei-Ning HAN
1
;
Yu XIA
;
Xue-Rong YA
;
Qiang SHEN
;
Hao PAN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; China; Evolution, Molecular; Genetic Variation; Hemagglutinin Glycoproteins, Influenza Virus; chemistry; genetics; immunology; Humans; Influenza A Virus, H1N1 Subtype; chemistry; classification; genetics; isolation & purification; Influenza, Human; virology; Molecular Sequence Data; Phylogeny; Sequence Homology, Amino Acid
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(4):266-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic variation and molecular characteristics of HA gene of influenza A (H1N1) virus isolated in Suzhou during from2009 to 2011.
METHODSViral RNA of 5 Suzhou isolates was isolated and their HA gene were amplified and sequenced by the primers and protocol recommended by WHO, and the sequences together with other sequences downloaded from GenBank were analyzed by several bioinformatics software.
RESULTSCompared with vaccine strain, the average homogeneity of nucleotide and amino acids of 5 Suzhou isolates were between 98.8-99.4% and 98.8-99.4% respectively. All of the 5 strains have 1 amino acids replacement in Sb region and 2 strains have 2 amino acids replacement in Ca region. Strains from and outside Suzhou both showed a trend of clustering by collection year.
CONCLUSIONThe Suzhou strains are still in stable condition although 1-2 amino acids replacement had happened in antigenic sites.
