Gene copy number, mRNA transcription and protein expression of PD-1 gene in primary hepatocarcinoma patients
10.3760/cma.j.issn.1003-9279.2012.04.013
- VernacularTitle:原发性肝癌患者PD-1基因拷贝数及其转录和蛋白表达水平的研究
- Author:
Hui-Min FAN
1
;
Ling-Jie WU
;
Feng-Yu HU
;
Zhan YANG
Author Information
1. 广州市第八人民医院
- Keywords:
Liver neoplasms;
Gene expression regulation,viral;
Apoposis
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(4):282-284
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the gene copy number,mRNA transcnption ano protien expression of programmed cell death 1 ( PD-1 ) gene in primary hepatocellular carcinoma (PHC) patients and normal control individuals ( NC ) who are anti-HBs positive,and to investigate the variations in PD-1 gene copy numbers and its relationship with PHC.Methods Real-time PCR was adopted to detect the PD-1 gene copy numbers and their mRNA expressions in peripheral blood mononuclear cells (PBMCs) from 24 samples of PHC patients and 26 of NC.Protein expression level of PD-1 on CD8+ T was analyzed by flow cytometry.Results In terms of number of PD-1 gene copy numbers,the percentage of cases of haploid (single) was 34.62％ and 4.17％ in PHC group and control group respectively while the percentage of cases of diploid (double) was 61.54％ and 95.83％ respoctively.The difference between the two was statistically significant (x2 =7.639,P =0.006).The rate of cases with double PD-1 gene copy numbers was found to be higher in patients with PHC than in control group.It was also found that the average expression of PD-1 mRNA was 2.35E-03 in control group and 1.23E-03 in PHC group.The expression level was significant lower in PHC group than that in control group when compared by using Mann-whitey technic ( U =153,P =0.009 ).Furthermore,the frequency of PD-1 protein expression on CD8+ T cells was 3.72 ± 0.32 in control group and 16.13 ± 1.68 in PHC group.The level of PD-1 mRNA expression was higher in PHC and significant differences was shown between two groups( t =- 7.073,P =0.000).Conclusions Our study suggests that the variation in PD-1 gene copy number may trigger primary hepatocellular carcinoma to HBV carriers.The relationship between the variation of PD-1 gene copy numbers and its association with primary hepatocellular carcinoma is worth further focus.
