Establishment of confirmatory test for suspicious hepatitis B surface antigen positive samples
10.3760/cma.j.issn.1003-9279.2012.04.022
- VernacularTitle:乙肝病毒表面抗原可疑阳性标本确认方法的建立
- Author:
Lin CHEN
1
;
Yang RONG
;
Jia LIU
;
Jun XU
;
Jing-Xia GUO
;
Yong-Ji SONG
;
Jing ZHAO
;
Ai-Xia LIU
;
Li-Hua YANG
;
Bo-An LI
;
Yuan-Li MAO
Author Information
1. 解放军第302医院
- Keywords:
Hepatitis B surface antigen;
Enzyme linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establish a confirmatory test based on ELISA,and use to verify the authenticity of HBsAg weak positive samples,pick and get rid of the false result,and avoid the mistake diagnosis.Method The particles (reagent A) coated by streptavidin and biotinylated HBsAb (reagent B)were mixed in different proportions,then neutralized with serum whose the COI of HBsAg ＞ 20 by ELISA in order to identify the activity of HBsAb in confirmatory reagent.30 pieces of HBsAg weak positive serum neutralized with the confirmatory reagent,the serum were considered to be positive if rate of decline of HBsAg COI ＞ 50％.The results were compared to Roche confirmatory Kit.Result Confirmatory reagent was able to neutralized with HBsAg.24 of 30 pieces of HBsAg weak positive samples were judged to be positive,while 6 pocces were negative.The ELISA comfirm method is fully consistent with Roche confirmatory Kit.Conclusion The ELISA confirmatory test for suspicious HBsAg positive samples is a simple,accurate and low cost initial validation method,After further clinical trials,should be widely applied.
