Comparison and analysis of two methods for serum free prostate-specific antigen (PSA) detection
10.3760/cma.j.issn.1003-9279.2012.04.024
- VernacularTitle:两种检测血清游离前列腺抗原方法的比较
- Author:
Yu-Qing JIANG
1
;
Zhi-Xian XIE
;
Qian LIU
;
Tian-Yi LIU
;
Zheng-Ran MA
;
Hao-Jia ZHI
Author Information
1. 卫生部北京医院
- Keywords:
Prostatic neoplasms;
Prostate-specific antigen;
Electrochemistry;
Chemiluminescence
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(4):316-318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the clinical performance of free prostate-specific antigen (fPSA)detection by ECLIA method,and evaluate whether ECLIA is suitable for clinical use.Methods 341samples were collected and tested prostate-specific antibodies with CMIA and ECLIA methods.These samples contain:97 samples with abnormal high PSA value tested by CMIA method,and 244 normal PSA samples.Use CMIA as the reference method,and detect fPSA,tPSA levels,and the ratio of fPSA/tPSA.Analyze the testing results with statistical methods.Results Compared with CMIA,correlation coefficent of ECLIA fPSA detection is 0.99; correlation coefficent of f/tPSA ratio detection is 0.96; the sensitivity,specificity of ECLIA f/tPSA ratio detection are 85.71％,92.6％ respectively,the agreement rate with ECLIA is 87.4％.No cross reaction with bilirubin,lipohemia,hemolysis,RF,CEA,AFP,CA125,CA153,CA199 were found in the tests.Conclusion The ECLIA method for free prostate-specific antigen detection showed good clinical performance; and is suitable for clinical use.
