Analysis of relationship of HBV PreS1 antigen, anti-HBc IgM , DNA load and genotypes in hepatitis B patients
10.3760/cma.j.issn.1003-9279.2012.05.003
- VernacularTitle:乙肝患者HBV感染指标、病毒复制水平与基因分型的关系分析
- Author:
Wang-Sen QIN
1
;
Li-Ping SHEN
;
Shuang ZHANG
;
Wen-Jiao YIN
;
Fing WANG
Author Information
1. 河南省人民医院检验科
- Keywords:
Hepatitis B;
Antigens;
Immunoglobulin M;
Genotypes;
Viral core proteins
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(5):328-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship of HBV PreS1 antigen,anti-HBc IgM,DNA load and genotypes,and the significance for clinical diagnosis and prognostic.Methods Enzyme linked immune-sorbent assay was used to test the HBV serum markers of HB patients; HBV-DNA copies was detected by time fluorescence quantitative PCR; using nested PCR to amplify the S fragment of HBV genome,then sequenc and make blast with HBV standard sequences to ascertain genotypes.Make comprehensive analysis of these indexes.Results 355 serum specimens of acute or chronic HB patients were collected.The positive rates of HBV PreS1-Ag and HBV-DNA in model Ⅰ (positive for HBeAg) were 80.2％ and 73.7％ respectively,which both higher than other models.The abnormal rate of ALT and AST were higher in PreS1-Ag positive group than negative,as well as in anti-HBc IgM positive group.There are 4 samples is genotype B (2.9％),76 genotype C(55.9％) and 56 genotype D (41.2％).Positive rate of HBeAg and HBV-DNA of genotype C samples were both higher than which of genotype B and D.Conclusion PreS1-Ag and Anti-HBe-IgM indexes are of great value to viral hepatitis B early diagnosis,HBV replication surveillance and prognostic evaluation; the major HBV genotypes in Henan province are C and D,and the positive rate of HBeAg and HBV-DNA were both higher in genotype C HBV infection population than genotype B and D.
