MicroRNA differential expression profiles in DC after the stimulation of HBsAg.
- Author:
Xiao-Jia XIONG
1
;
Yan-Zhong PENG
;
Xing-Fei PAN
;
Qi-Huan XU
;
Gang LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Dendritic Cells; drug effects; metabolism; Gene Expression Profiling; Hepatitis B Surface Antigens; pharmacology; Lipopolysaccharides; pharmacology; Male; Mice; Mice, Inbred C57BL; MicroRNAs; analysis; Oligonucleotide Array Sequence Analysis; Tumor Necrosis Factor-alpha; pharmacology
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(5):331-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the differential expression profiles for exploring new immune mechanism of hepatitis B virus infection.
METHODSDCs were separated from the bone marrow of healthy mouse and cultured in vitro. Then DCs were stimulated with HBsAg, LPS, TNF-alpha, respectively. Twenty-four hours later, the total RNA of cell was extracted. cDNA microarray was used to compare the differential expression of RNA. The significant differential expression of miRNA after the stimulation of HBsAg was chosen. Subsequently, target genes of the significant differential miRNA were forecasted by using computer software.
RESULTSThere were 30 miRNAs whose significant differential expressions were beyond two times after the stimulation of HBsAg. Among them, 16 miRNAs expressions were increased and 14 miRNAs expressions were decreased. There was significant difference in differential expression of miRNA among the 3 different stimulations. The selected target genes included relevant elements of signal pathway of DC.
CONCLUSIONThe alteration of expression profiles of miRNA was specific after DC was stimulated by HBsAg. The selected target genes further demonstrated that miRNA could play important roles in the immune mechanism of HBV infection.
