Effect of gene optimization on the expression and purification of HDV small antigen produced by genetic engineering
10.3760/cma.j.issn.1003-9279.2012.05.005
- VernacularTitle:基因优化对基因工程丁型肝炎小抗原蛋白表达和纯化的影响
- Author:
Jun-Ying DING
1
;
Qing-Ling MENG
;
Min-Zhuo GUO
;
Yao YI
;
Qiu-Dong SU
;
Xue-Xin LU
;
Feng QIU
;
Sheng-Li BI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Genes;
Hepatitis D;
Expressed sequence tags
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(5):335-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of gene optimization on the expression and purification of HDV small antigen produced by genetic engineering.Methods Based on the colon preference of E.coli,the HDV small antigen original gene from GenBank was optimized.Both the original gene and the optimized gene expressed in prokaryotic cells,SDS-PAGE was made to analyze the protein expression yield and to decide which protein expression style was more proportion than the other.Furthermore,two antigens were purified by chromatography in order to compare the purity by SDS-PAGE and Image Lab software.Results SDSPAGE indicated that the molecular weight of target proteins from two groups were the same as we expected.Gene optimization resulted in the higher yield and it could make the product more soluble.After chromatography,the purity of target protein from optimized gene was up to 96.3％,obviously purer than that from original gene.Conclusion Gene optimization could increase the protein expression yield and solubility of genetic engineering HDV small antigen.In addition,the product from the optimized gene group was easier to be purified for diagnosis usage.
