Isolation and sequence analysis on virus of HFMD with encephalitis in Yantal city.
- Author:
Wen-qing HAN
1
;
Lian-Feng GONG
;
Juan LIU
;
Ping ZHANG
;
Qiao GAO
;
Ying SUN
;
Li-Qing LV
;
Hong MEMG
Author Information
- Publication Type:Journal Article
- MeSH: Capsid Proteins; genetics; Encephalitis, Viral; virology; Enterovirus A, Human; classification; genetics; isolation & purification; Hand, Foot and Mouth Disease; complications; virology; Humans; Phylogeny
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(5):338-340
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city.
METHODSStool samples and cerebrospinal fluids (CSF) collected from HFMD with encephalitis cases in Yantai. Virus were isolated from stool samples and identified by fluorescence reverse transcription-polymerase chain reaction. The VPl region was amplified and sequenced from positive specimens. Genetic characterization was identified by sequence analysis.
RESULTSGetting 3 virus strains from 10 stool specimens and all of them belong to EV71. The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98%-99% and 98.90%-99.45% respectively.
CONCLUSIONThe pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.
