Isolation and sequence analysis on virus of HFMD with encephalitis in Yantai city
10.3760/cma.j.issn.1003-9279.2012.05.006
- VernacularTitle:烟台市手足口病合并脑炎病毒分离及基因序列分析
- Author:
Wen-Qing HAN
1
;
Lian-Feng GONG
;
Juan LIU
;
Ping ZHANG
;
Qiao GAO
;
Ying SUN
;
Li-Qing LV
;
Hong MEMG
Author Information
1. 264003,烟台市疾病预防控制中心
- Keywords:
Hand,foot and mouth disease;
Encephalitis;
Enterovirus;
Sequence analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(5):338-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city.Methods Stool samples and cerebrospinal fluids (CSF) collected from HFMD with encephalitis cases in Yantai.Virus were isolated from stool samples and identified by fluorescence reverse transcription-polymerase chain reaction.The VP1 region was amplified and sequenced from positive specimens.Genetic characterization was identified by sequence analysis.Results Getting 3 virus strains from 10 stool specimens and all of them belong to EV71.The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98％-99％ and 98.90％-99.45％ respectively.Conclusion The pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.
