Comparison of prediction performance of PAHs carcinogenicity between a BALB/c-E6E7 cell transformation assay and a BALB/c 3T3 cell transformation assay
10.3760/cma.j.issn.1003-9279.2012.05.012
- VernacularTitle:两种检测多环芳烃致癌性细胞转化实验方法的研究
- Author:
Shuang WU
1
;
Jin-Tao LI
;
Ru-Gang ZHONG
;
Yi ZENG
Author Information
1. 100124,北京工业大学生命科学与生物工程学院
- Keywords:
Cell line;
Carcinogenecity tests;
Cell transformation,viral
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(5):359-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To predict the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs) by cell transformation assay using BALB/c 3T3 cells and HPV16-E6E7-transfected BALB/c 3T3 cells (BALB/c-E6E7 cells).Methods The cell transformation assays induced by PAHs using BALB-E6E7 cells and BALB/c 3T3 cells.Results The initiating and promoting activities of PAHs examined in a BALB-E6E7 cell transformation assay were similar to in a BALB/c 3T3 cell transformation assay,which was up to the standard of agents classified by the IARC.There were much more transformed foci appeared and much shorter time consumed to accomplish phenotypic alterations in the BALB/c-E6E7 cell transformation assay than in the BALB/c 3T3 cell transformation assay.The BALB/c-E6E7 cell transformation assay was superior to the BALB/c 3T3 cell transformation assay in cost and labor performance,the sensitivity of transformation response.Conclusion The BALB/c-E6E7 cell transformation assay,with a satisfied prediction performance of initiating activity and promoting activity,would improve the overall process of safety and risk assessment of carcinogenicity.
