The influence of representative herbs of clearing and detoxifying drugs effect on inflammatory cytokines expression of mice lung homogenate infected by influenza virus FM1
10.3760/cma.j.issn.1003-9279.2012.06.005
- VernacularTitle:黄芩、板蓝根等清热解毒药对流感病毒所致肺炎小鼠炎性因子蛋白表达的影响
- Author:
Cheng-Xiang WANG
1
;
Hong-Yun CAO
;
Miao CHENG
;
Hong-Ri XU
;
Ming-Zhe WANG
;
Li-Li XU
;
Hui-Yong YU
;
Qian LI
Author Information
1. 100700,北京中医药大学东直门医院
- Keywords:
Drugs,Chinese herbal;
Influenza A virus;
Cytokines;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(6):415-418
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to screen out a certain kind of traditional medicine which has a better role in immune regulatory,the influence of representatives of heat clearing and detoxicating herb on inflammatory cytokines protein expression of mice lung homogenate infected by FM1 have been observed.Method Modeling mice infected by FM1.On the first,third,fifth and seventh day after FM1 infection,tumor necrosis factor-α (TNF-alpha),interleukin-1 (IL-1),interleukin-6 (IL-6),interleukin-1 0 (IL-10),and γ-interferon (IFN-γ) expression in mice lung homogenate of normal control group,model control group,scutellari group,isatidis group,pulsatilla group,polygonum cuspidatum group and oldenlandia group have been tested by ELISA method.Result The expression of TNF-α,IL-6,IFN-γ and IL-10 in mice lung homogenate reaches its peak on the third day after FM1 infection,significantly higher than the control group (P ＜ 0.05).Scutellari and isatidis are two representatives of heat clearing and detoxicating herb,which can decrease the expression of TNF-α,IL-6 and IL-1 and increase the expression of IL-10,IFN-γ.The effect are more pronounced and statistically significant (P ＜ 0.05) on the third and fifth day after infection,pulsatilla,polygonum cuspidatum and oldenlandia can also regulate the inflammatory cytokines,but the effect are not so obvious as scutellari and isatidis.Conclusion Scutellari and isatidis,two representatives of heat clearing and detoxicating herb,have a good intervention on immune damage caused by influenza virus through adjusting the balance of inflammatory cytokines and anti-inflammatory cytokines.
