The genetic stability of recombinant adenovirus expressing human rotavirus VP6 gene which used Ad41 as vector.
- Author:
Min WANG
1
;
Duo-Ling CHEN
;
Jing-Dong SONG
;
Jian-Guo QU
;
Zhuo-Zhuang LU
;
Wei-Ming ZHAO
;
Tao HONG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Antigens, Viral; genetics; metabolism; Capsid Proteins; genetics; metabolism; Cell Line; Gene Expression; Genetic Vectors; genetics; metabolism; Humans; Rotavirus; genetics; metabolism; Rotavirus Infections; virology
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):422-424
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic stability of non-replicating recombinant adenovirus which used Ad41 as vector and could express VP6 gene of group A rotavirus during continous passage, in order to develop the vaccine of rotavirus.
METHODSThe recombinant adenovirus rvAd41-VP6 (o) was prepared by our laboratory early, it then was continuously propagated on 293TE7 cells for 14 passages. After that samples of the infected cells were collected at every 2 passages for the detection of the integration of the VP6 gene by PCR, and the expression of the target protein was detected by Western Blot analysis.
RESULTSAnalysis by PCR revealed that, there was stable integration of specific VP6 gene in the rvAd41-VP6 (o), Western Blot analysis confirmed that rvAd41-VP6 (o) could stably expressed the group-specific antigen structural protein VP6 (o), and it had preferable genetic stability.
CONCLUSIONThe recombinant adenovirus rvAd41-VP6 (o) which could stably express the VP6 (o) gene had favorable biological property in vitro, and it has provided a basis for further research of animal immunization.
