Study on the correlation between membrane protein Flotillin-1 and PrPc endocytosis.
- Author:
Ke REN
1
;
Ke WANG
;
Yin XU
;
Long-Zhu LI
;
Jin ZHANG
;
Hui WANG
;
Yu-E YAN
;
Xiao-Ping DONG
;
Chen GAO
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Membrane; genetics; metabolism; Endocytosis; Humans; Membrane Proteins; genetics; metabolism; PrPC Proteins; genetics; metabolism; Protein Binding; Protein Transport
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):435-438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPc.
METHODSThe expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPc in Cells which were treated with copper ions was observed using immunoprecipitation method.
RESULTS(1) Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; (2) Only in the appearance of copper ions, the protein complexes of PrPc and Flotillin-1 can be detected with the method of IP, which were related to copper ions concentration and processing time.
CONCLUSIONThe membrane-associated protein Flotillin-1 has the relationship with the endocytosis of PrPc.
