Study on the correlation between membrane protein Flotillin-1 and PrPC Endocytosis

VernacularTitle:膜蛋白Flotillin-1与细胞膜PrPC内吞相关性研究
Author: Ke REN ¹ ; Ke WANG ; Yin XU ; Long-Zhu LI ; Jin ZHANG ; Hui WANG ; Yu-E YAN ; Xiao-Ping DONG ; Chen GAO
Author Information

1. 中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室
Keywords: Prions; Viral matrix proteins; Endocytosis; Copper
From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):435-438
CountryChina
Language:Chinese
Abstract: Objective To explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPC.Methods The expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPC in Cells which were treated with copper ions was observed using immunoprecipitation method.Results ①Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; ②Only in the appearance of copper ions,the protein complexes of PrPC and Flotillin-1 can be detected with the method of IP,which were related to copper ions concentration and processing time.Conclusion The membraneassociated protein Flotillin-1 has the relationship with the endocytosis of PrPC.