The comparison between HIV-infected patients' Vdelta2 T cells expansion efficiencies by zoledronic acid and gammadelta TCR monoclonal antibody in vitro
10.3760/cma.j.issn.1003-9279.2012.06.013
- VernacularTitle:唑来膦酸与γδ TCR单抗体外扩增HIV感染者Vδ2 T细胞的效果比较
- Author:
Wen XU
1
;
Xiao-Li WU
;
Yang-Xin XIE
;
Jun-Lian YANG
;
Wei-Min NIE
;
Min ZHAO
;
Wei-Wei CHEN
Author Information
1. 解放军第三○二医院感染性疾病诊疗与研究中心
- Keywords:
HIV;
T-Lymphocytes;
Phosphonce acid
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(6):442-445
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out the more efficient induction method through investigating the expansion efficiencies of HIV-infected patients' Vdelta2 T cells induced by zoledronic acid (Zol) or gammadelta TCR monoclonal antibody (mAb).Methods 38 healthy control subjects (HC group) and 65 HIV infected patients (HIV group) were enrolled in this research.Peripheral blood mononuclear cells (PBMCs) of individuals were stimulated by Zol or γδ TCR mAb respectively for 14 days at 2.0 × 107 cells/well,and then γδ T cells and Vδ2 subsets frequencies were measured by flow cytometry (FCM) on 0,7 and 14 day.The absolute numbers of Vδ2 T cells were calculated and the Vδ2 T cell expansion efficiencies by these two methods were compared.Results The absolute numbers and frequencies of Vδ2 T cell of HIV groups were lower than those of HC groups significantly on 0 day.After 14 days,the frequencies of Vδ2 T cell of HIV group and HC group were(17.6 ± 19.8)％ and(64.3 ± 4.5) ％ respectively,and the expansion indexes of Vδ2 T cell were 54 ± 40 and 74 ± 29 respectively by induction of γδ TCR mAb.However,the frequencies of Vδ2 T cell of HIV group and HC group were(69.6 ± 21.2)％ and(97.3 ± 1.7)％ respectively,and the Vδ2 T cell expansion indexes were 538 ± 11 and 5984 ±721 respectively by induction of Zol.Conclusion Zol could induce the vast expansion of Vδ2 T cells of HIV infected patients.The expansion efficiency by Zol was better than that by the γδ TCR mAb.
