Mutation analysis of the HBV reverse transcriptase in nucleos(t)ide-treated patients with chronic HBV infection.
- Author:
Xiu-Juan JIAO
1
;
Xun PENG
;
Xiu-Min JIAO
;
Jin-Sheng WANG
;
Xie-Wen SUN
;
Pei-Li ZHAO
;
Shou-Yun WANG
;
Jia-Qun LIU
;
Tong LI
;
Jing-Xian YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Antiviral Agents; therapeutic use; Female; Hepatitis B virus; drug effects; enzymology; genetics; Hepatitis B, Chronic; drug therapy; virology; Humans; Male; Middle Aged; Mutation; drug effects; Nucleosides; therapeutic use; Nucleotides; therapeutic use; RNA-Directed DNA Polymerase; genetics; metabolism; Viral Proteins; genetics; metabolism; Young Adult
- From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):453-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment.
METHODSSerum samples of 229 CHB patients with NA treatment were obtained. Full-length HBV RT sequences were amplified, sequenced and analyzed, on the following NA resistant (NAr) mutations belonging to different NAr pathways.
RESULTSAmong 229 HBV isolates, 14.41% (33/229) and 85.59% (196/229) were genotype B and C, respectively; and the patients with HBV genotype C may be more susceptible to develope resistant mutations than patients with HBV genotype B(chi2 = 2.95, P < 0.05). NAr mutations were detected in 63 CHB patients. Mutations were not found at rtI169, rtT184, rtA194 or rtS202. RtM204 mutations were detected at the highest frequency among 63 mutants (40/63, 63.49%) and found to display 11 combination mutation patterns, in which rtM204I were associated with rtL80I/V and rtL180M, and rtM204V were associated with rtL1l80M, respectively. Conclusions There are complicated mutation patterns in the HBV RT region for chronic hepatitis B (CHB) patients with nucleos(t)ide analogue (NA) treatment. RtM204V/I mutation was the highest.
