Hepatitis A virus mimotope mapping by phage display peptide library
10.3760/cma.j.issn.1003-9279.2012.06.017
- VernacularTitle:用噬菌体展示肽库技术筛选甲肝病毒抗原模拟表位
- Author:
Jing-Yuan CAO
1
;
Jian-Dong LI
;
Hui-Hui ZHENG
;
Sheng-Li BI
;
De-Xin LI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatitis A virus;
Peptide library;
Epitopes
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(6):456-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective A 12 mer phage display peptide library was used to identify hepatitis A virus mimotopes of antigenic determinants,to provide the feasibility of virus epitope mapping by using this approach.Methods Using purified anti-hepatitis A virus monoclonal antibody as affinity selective molecule,phage display peptide library was biopanned and positive clones were selected by ELISA,competition assay and DNA sequencing.Results 10 ELISA positive clones were chosen for DNA sequencing,and the displayed peptide sequences were deduced.9 of them showed identical nucleotide sequence,and similarity in their amino acid sequence with VP1 of HAV HM175 was found,but no sequence homology was found between the other phage clone and the capsid proteins of HAV.Those peptides may behave as mimotopes of HAV.Conclusion The mimotope of HAV was selected by using phage display peptide library screening.The results provide the potential of this method to search for the mimotopes of the virus.
