Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay
10.3760/cma.j.issn.1003-9279.2012.06.025
- VernacularTitle:一种改良的环介导恒温扩增技术在甲型肝炎病毒核酸检测上的应用
- Author:
Feng QIU
1
;
Min-Zhuo GUO
;
Jun-Ying DING
;
Yao YI
;
Zhi-Yuan JIA
;
Qiu-Dong SU
;
Sheng-Li BI
Author Information
1. 中国疾病预防控制中心病毒病预防控制所
- Keywords:
Hepatitis A virus;
Nucleic acid amplification technique;
Sensitivity and specificity
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(6):483-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a novel improved loop-mediated isothermal amplification (LAMP) technique to detect hepatitis A virus (HAV).Methods A novel improved LAMP assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection.Results Precision and reproducibility analysis proved its high stability and reliability.Comparison between the improved and conventional LAMP assays revealed that the former was more sensitive with a detection limit of 5 TCID5o/ml.The novel detection method displayed 100％ consistency with the TaqMan real-time PCR assay when applied to clinical specimens collected from patients with confirmed acute HAV infection.Conclusion This novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.
