Development and evaluation of a quantitative double antibodies sandwich ELISA assay for rIFN-α1b

VernacularTitle:双抗体夹心ELISA定量检测重组人干扰素α1b方法的建立与评价
Author: Mei-Ying WU ¹ ; Yan-Ping AI ; Yan CAO ; Shuang WU ; Xiao-Xia NIU ; Yong-Qing CHENG
Author Information

1. 北京三元基因工程有限公司
Keywords: Quantitative detection; Interferon-alpha; Enzyme-linked immunosorbent assay
From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):489-491
CountryChina
Language:Chinese
Abstract: Objective To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon α1b.Methods Mouse monoclonal antibodies with different binding site on rIFN-α1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively.And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit,specificity,accuracy and reproducibility were evaluated and validated.Results The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml,with a liner detection range 10-100 ng/ml (R2 =0.992),variation coefficient inter-plates is less than 10％.Conclusion The developed sandwich ELISA was a sensitive and specific,accuracy and reproducibility method for quantitative determination of recombinant human interferon αt1b in final product.