Comparison of two different methods to detect HIV antibodies

VernacularTitle:两种不同方法检测HIV抗体结果的比较
Author: Jing-Xia GUO ¹ ; Jun XU ; Lin CHEN ; Jia LIU ; Jing ZHAO ; Yong-Ji SONG ; Ai-Xia LIU ; Bo-An LI ; Yuan-Li MAO
Author Information

1. 解放军第三○二医院临床检验中心
Keywords: HIV; Enzyme-linked immunosorbent assay; Chemiluminescence method
From: Chinese Journal of Experimental and Clinical Virology 2012;26(6):492-493
CountryChina
Language:Chinese
Abstract: Objective Evaluated the chemiluminescence and enzyme-linked immunosorbent assay (ELISA) to detect HIV antibodies,and compared the results,to provide a reference for the selection and clinical application of HIV screening.Methods 3000 cases of our hospital patients were measured by enzyme-linked immunosorbent assay and chemiluminescence immunoassay,using comfirmming experimental results as gold standards.Comparing sensitivity,specificity and other Indicators.Results In the diagnosis of HIV infection,enzyme-linked immunosorbent assay and chemiluminescence immunoassay had no significant difference.The positive rate of enzyme-linked immunosorbent assay was 0.93％,while the sensitivity and specificity were 89.66％,99.93％,the positive rate of chemiluminescence immunoassay was 1.03％,while the sensitivity and specificity were 100％,99.93％,respectively.Conclusion Both methods are suitable for screening of HIV,having high specificity,and chemiluminescence has greater sensitivity than ELISA.